Campylobacter Blood-Free Selective Agar (CCDA)

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Code: KM0057-500G

Variation ID		Product Code	Pack Size	List Price	Qty
8234	photo_camera	KM0057-500G	500g KM0057-500G	£88.80

Pack: 500g

£88.80

Campylobacter Blood-Free Selective Medium (CCDA) is one of several media formulations available for the selective isolation of Campylobacter spp., primarily C. jejuni and C. coli. CCDA was described by Bolton et al. and formulated to replace blood with a combination of charcoal, ferrous sulphate, and sodium pyruvate.

CCDA is recommended for food testing. CCDA with the addition of yeast extract and cefoperazone is used in the isolation of Campylobacter spp. from foodstuffs and swabs in the FDA/BAM method.

This product complies with the requirements of ISO 10272-1:2006.

Bolton et al. recommended incubating inoculated plates at 37C to improve isolation rates but incubation at 41.5C is recommended for the isolation of the commonly encountered thermophilic species (C. jejuni and C. coli).

The meat peptone, beef extract and tryptone provide the required nitrogen, carbon and vitamins. Bacteriological charcoal absorbs toxic compounds and metabolites. Ferrous sulphate and sodium pyruvate are oxygen scavengers. Sodium desoxycholate is a selective agent. Through the addition of campylobacter (Preston) supplement (LS0010), which consists of cefoperazone and amphotericin B, enteric flora is suppressed.

Used in the isolation of Campylobacter spp. from foodstuffs and swabs in the FDA/BAM method.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Black

Prepared Medium Colour: Black

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Recommended Incubation: At 41.5±1°C for 40-48 hours under micro-aerophilic conditions

Formula*g/L