APC Pure Logo webp
shopping_cart 0 search menu
Deoxycholate Citrate Agar (DCA) Hynes Group Picture

Deoxycholate Citrate Agar (DCA) Hynes

Excl. VAT Incl. VAT
Code: KM0050-500G
  Pack Size List Price Qty  
photo_camera 500g
KM0050-500G
£87.30
Pack: 500g

£87.30

This is a selective medium for the isolation of Salmonella spp. and Shigella spp. from clinical specimens and environmental samples.

This formulation was developed by Hynes through a modification of Leifsons DCA medium.

The peptone provides the required carbon, nitrogen and vitamins. Lactose is a fermentable carbohydrate and neutral red is a pH indicator. Sodium thiosulphate and ferric citrate are used to detect hydrogen sulphide production indicated by the black centred colonies of hydrogen sulphite positive organisms.

Sodium desoxycholate and sodium citrate inhibit most Gram-positive organisms.

Used for the isolation of Salmonella spp. and Shigella spp. from clinical specimens and environmental samples.

Additional Information

Shelf Life (days): 1095 days

Storage Temp (C): 10-30°C

Dehydrated Medium Appearance: Fine Powder

Prepared Medium Appearance: Firm Gel

Dehydrated Medium Colour: Straw

Prepared Medium Colour: Pale orange/pink

Dehydrated PH: 7.4±0.2

Prepared PH: 7.4±0.2

Formula*g/L

Peptone: 10.0

Lactose: 10.0

Sodium desoxycholate: 5.0

Sodium citrate: 8.5

Sodium thiosulphate: 5.4

Ferric citrate: 1.0

Neutral red: 0.02

Agar: 12.0

Total: 52.0

*Adjusted/supplemented as required to meet performance requirements

You may also like...