Variation ID | Product Code | Pack Size | List Price | Qty | ||
---|---|---|---|---|---|---|
photo_camera | KM0111-500G | 500g
KM0111-500G |
£83.40 |
Kligler iron agar is used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.
Kligler iron agar is a modification of the original formulation developed by Kligler.
It incorporates the principles of Russells double sugar agar with Kligler s lead acetate agar.
The peptone provides the required nitrogen, carbon and vitamins. Lactose and glucose are carbohydrates.Acid production from their fermentation is detected by the phenol red pH indictor. Sodium thiosulphate is reduced to hydrogen sulphide which is detected by the ferric citrate indicator.Sodium chloride maintains the osmotic balance.
Used to differentiate between some of the enterobacteriacae on the basis of three reactions: fermentation of lactose and glucose and the production of hydrogen sulphide.
Additional Information
Shelf Life (days): 1095 days
Storage Temp (C): 10-30°C
Dehydrated Medium Appearance: Fine Powder
Prepared Medium Appearance: Firm Gel
Dehydrated Medium Colour: Straw
Prepared Medium Colour: Red
Dehydrated PH: 7.4±0.2
Prepared PH: 7.4±0.2
Recommended Incubation: Aerobically at 35±2°C for 18-24 hours
Formula*g/L
Peptone: 20.0
Sodium chloride: 5.0
Lactose: 10.0
Glucose: 1.0
Ferric citrate: 0.5
Sodium thiosulphate: 0.3
Phenol red: 0.025
Agar: 12.0
Total: 49.0
*Adjusted/supplemented as required to meet performance requirements