Variation ID | Product Code | Pack Size | List Price | Qty | ||
---|---|---|---|---|---|---|
photo_camera | GPC8038-X | 100g
GPC8038-X |
£8.97 | |||
photo_camera | GPC8038-2X | 250g
GPC8038-2X |
£15.88 | |||
photo_camera | GPC8038-5X | 500g
GPC8038-5X |
£27.48 | |||
photo_camera | GPC8038-Y | 1Kg
GPC8038-Y |
£97.95 |
What is Tris? Tris (Tris(hydroxymethyl)aminomethane) (chemical formula C₆H₁₉NO₃) is an organic compound commonly used as a buffering agent in biological and chemical research. It appears as a white crystalline powder that is highly soluble in water, making it ideal for use in a variety of aqueous solutions. Tris is widely employed in molecular biology, biochemistry, and laboratory applications due to its ability to stabilise the pH of solutions. It is also known by alternate names such as Tris Base or THAM (tris(hydroxymethyl)aminomethane).
Consistent Quality: We provide only high-quality Tris 99.0%, ensuring reliable and consistent performance in laboratory, pharmaceutical, and industrial applications.
Shipped from Manchester, UK Facility: Sourced and packaged in non-toxic, chemical-resistant containers to ensure safe handling and preserve product quality during transport.
Frequent Uses
Buffering Agent in Biochemistry
Widely used as a buffering agent in biochemistry to maintain a stable pH in various biochemical assays. It is particularly effective in biological experiments that require a neutral pH, such as DNA/RNA extraction, electrophoresis, and enzyme reactions. Tris helps prevent changes in pH that could interfere with the activity of biomolecules.
Molecular Biology
An essential component of many buffer solutions, including those used in DNA and RNA analysis. It is used in the preparation of TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) buffers for gel electrophoresis, as well as in PCR (Polymerase Chain Reaction) and DNA sequencing. Its ability to stabilise pH is crucial for the accuracy of these techniques.
Protein Chemistry
Often used in protein chemistry to maintain the pH of solutions during protein purification, enzyme assays, and the preparation of protein samples for analysis. Its buffering capacity ensures that proteins remain stable and active throughout the analysis process.
Pharmaceutical Applications
As an excipient in the formulation of injectable solutions, particularly in vaccines and other biologic products. It helps maintain the pH of these formulations, ensuring their stability and effectiveness over time.
Electrophoresis
Widely employed in electrophoresis, a technique used to separate macromolecules such as proteins, nucleic acids, and lipids based on their size and charge. Tris-containing buffers are commonly used in gel electrophoresis for both protein and DNA separation, as its pH-stabilising properties ensure accurate and reproducible results.
Cell Culture Media
Frequently used in cell culture media to maintain the pH of the growth medium, supporting the optimal growth and viability of cultured cells. It is a key ingredient in solutions that facilitate the growth of mammalian, bacterial, and plant cells in laboratory settings.
Biological Buffers
In the preparation of biological buffers, which are essential for maintaining the physiological pH of body fluids and tissue samples. These buffers are used in various research applications, including clinical diagnostics and in-vitro studies.
Minimum Assay: >99.0%
Molecular Formula: C4H11NO3
Molecular Weight: 121.14
Melting Point: 168 - 172 deg C
Appearance: White Microcrystalline Powder
Heavy Metals: <5ppm
Iron (Fe): <1ppm
Residue on Ignition: <0.1%
pH (5% Aqueous Solution): 10.0-11.5
Cas Number: 77-86-1
Einecs Number: 201-064-4
HS Code / Commodity Code: 2922190090
Hazard Phrases: H315,H319,H335
Prec Phrases: P261,P280,P302+P352,P305+P351+P338